EX VIVO PRODUCED ORAL MUCOSA EQUIVALENT BY USING THE DIRECT EXPLANT CELL CULTURE TECHNIQUE
نویسندگان
چکیده
منابع مشابه
Ex vivo produced oral mucosa equivalent by using the direct explant cell culture technique.
OBJECTIVE The aim of this study is the histological and immunohistochemical evaluation of ex vivo produced oral mucosal equivalents using keratinocytes cultured by direct explant technique. MATERIAL AND METHODS Oral mucosa tissue samples were obtained from the keratinized gingival tissues of 14 healthy human subjects. Human oral mucosa keratinocytes from an oral mucosa biopsy specimen were di...
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The objective of this study was to assess the efficacy of the use of an ex vivo produced oral mucosa equivalent (EVPOME) for intraoral grafting procedures. Autogenous keratinocytes were harvested from a punch biopsy 4 weeks prior to surgery, placed in a serum-free culture system and seeded onto a human cadaveric dermal equivalent, AlloDerm. Thirty patients with either a premalignant or cancerou...
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Introduction: A reliable source of cultured keratinocytes is essential as a component of oral mucosa substitutes to treat burns and wounds of the oral and maxillofacial region. Primary monolayer cell cultures have been also extremely helpful in the study of the basic biology and responses to stimuli, of both oral and skin keratinocytes, and many studies have used them. There are two techniques ...
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PURPOSE We sought to develop full-thickness ex vivo produced human conjunctiva and oral mucosa equivalents using a serum-free culture system without a feeder layer and to compare conjunctiva and oral mucosa equivalents to assess their suitability as graft materials for eyelid reconstruction. MATERIALS AND METHODS Human conjunctival and oral mucosal keratinocytes were cultured, expanded, and s...
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PURPOSE We have fabricated, for clinical application, artificial oral mucosa that totally excludes both heterogenic protein interaction and xenotransplantation. The purpose of this study is to compare the fatty acid composition of cell membrane phospholipids related to post-transplantation epithelial regeneration. MATERIALS AND METHODS Cultured keratinocytes, keratinocytes at 2, 3, 4, and 9 w...
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ژورنال
عنوان ژورنال: Balkan Medical Journal
سال: 2012
ISSN: 2146-3131,2146-3131
DOI: 10.5152/balkanmedj.2012.011